FPLC uses several purification methods including ion-exchange, gel filtration, and affinity as well as other lesser used methods. FPLC purification is usually a multi-step process including a combination of methods. The columns also differ as well as the column materials and particle sizes. The HPLC columns are made out of stainless steel. Most HPLC resins are made of silica beads with small particle sizes that can stand very high back pressures.
FPLC uses agarose, polymer materials, or silica materials. The particle sizes for FPLC are larger and have large pore sizes. The FPLC resins are not as capable of handling very high back pressures due to the previously mentioned material and sizes. HPLC typically uses stainless steel in the flow path.
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